Journal: bioRxiv

Article Title: METTL16 promotes taxane resistance in Triple-Negative Breast Cancer through m 6 A-dependent translational upregulation of ABCB1

doi: 10.64898/2026.03.11.710933

Figure Lengend Snippet: A. Recombinant protein biochemical methylation assay (MTase-Glo TM ) showing that recombinant WT METTL16 exhibits robust, dose-dependent m 6 A methylation activity toward the MAT2A substrate, whereas the catalytically inactive N184A mutant displays markedly reduced activity, as measured by background-corrected luminescence. B. MDAMB231 cells were infected with empty vector or lentiviral constructs encoding WT METTL16 (WT ME) or the N184A point mutation of METTL16. Upper panel: The level of METTL16 protein was determined by Western analysis of total cell lysates. GAPDH was used as a control. Lower panel: Cell survival assays were performed after treatment with paclitaxel at different doses. The fraction of surviving cells was determined by normalizing the data from paclitaxel treated cells to DMSO controls. **, P< 0.01; ***, P< 0.001.

Article Snippet: Lentiviral vectors (lenti-sgRNA hygro, Addgene # 104991) containing METTL16-targeting sgRNA (sgME) or control sgRNA (sgNS) were kindly provided by Drs. Rui Su and Jianjun Chen (Beckman Research Institute of City of Hope, Monrovia, CA).

Techniques: Recombinant, Methylation, Activity Assay, Mutagenesis, Infection, Plasmid Preparation, Construct, Western Blot, Control